This study examines the interpretation of the Qur'an using the Javanese tembang method in Gus Ali Muttaqin Purwodadi's recitation. The purpose of this research is to find out the meaning of denotation, connotation and myth in the tembang’s that have been described in Surah Ali-Imran verse 185, An-Nahl verse 123, Ar-Rum verse 2 and Al-Baqarah verse 172. Javanese tembang become a tool used by Gus Ali to explain the meaning of the content of the Qur'an. The poems in the tembang are designed in such a way according to the theme of the study. This study uses semiotic analysis by Roland Barthes, being able to answer some problems related to the meanings studied. Furthermore, the steps used in this study are: first, looking for the original meaning by translating the tembang into Indonesian, second, researching some words that can represent connotative meanings, third, analyzing the data has been obtained to get messages (myth) conveyed in these verses. The existence of this research will be able to contribute to the development of science in the field of semiotics of the interpretation of the Qur'an. In addition, the discussion studied also has a close relationship with Indonesian culture in the form of Javanese tembang’s.