Optimisation of salmon oil hydrolysis with papaya lipase
Abstract
The process of DHA and EPA enrichment for fish oil by enzyme includes several steps, among which hydrolysis of fish oil with non-specific lipase is usually used as the first step. In this study, the results of fish oil composition analysis by GC before and after the hydrolysis show that lipase from Carica papapa latex (CPL) was non-specific lipase and can be exploited in the first step of DHA and EPA enrichment procedure. Using designed experiment of Box-Hunter model, the optimized conditions of fish oil hydrolysis with CPL are determined as follows: temperature of 29oC and pH of 7.6; water/oil ratio of 1.36:1 and enzyme level of 1.34%. This study will be used as the essential step in application of CPL in DHA and EPA enrichment of salmon oil to produce functional food.
Moraref