The effect of drought on protein profiles and activities of enzymes of germinating seeds and seedlings of vigna radiata of DX 14 and DX 208
Abstract
Drought is one of the major abiotic stresses that reduce crop productivity. Drought leads to many physiological and biochemical changes of plants related to stress tolerance. The purpose of this study is to determine the variation of protein profiles and activity of some enzymes in the germination and seedling stage of two varieties of mung bean DX 208 and DX 14 after 3, 5 and 7 days of drought treatment. Electrophoresis results on SDS polyacrylamide gel show that the new protein with a molecular weight of about 50 kDa is activated after 5 days and 7 days of drought treatment in both DX 208 and DX 14. In addition, DX 14 also appears a protein group of about 45 kDa after 7 days of drought. In the condition of dehydration, the activity of amylase and lipase of germinated seeds and leaves increase compared to the control. Particularly, DX 14 increases more than DX 208; the catalase activity is significantly reduced over drought treatment time, in which the variety of DX 208 decreases more than DX 14.
Moraref