Seven microalgae strains from samples collected from freshwater lakes in Central Vietnam were isolated and cultivated in BBM medium for investigating their biological characteristics and their applicability. These strains were identified belonging to 6 species, 3 genera of the family Scenedesmaceae, division Chlorophyta. Their average growth rate was from 0.081 - 0.160 d-1, and the highest cell density of each strain reached from 2.6 x 106 - 60 x 106 cells/ml. The total content of pigments (including chlorophyll-a, chlorophyll-b, and carotenoid) of all strains was not too high (4.10 - 11.3 μg/mL), but the total lipid content exhibiting noticeably high levels, accounted for 16.6% - 74.1% of dry cell weight. Among isolated strains, Desmodesmusabundans and D.costato-granulatus were the most prominent with the highest growth rate and density in combination with remarkably high contents of lipids and pigments, and thus were identified as two potentially great candidates for biofuel production and chlorophyll and carotenoid extraction.