ABSTRACT Background: Collagen has vital role on healing process of burn injuries, mainly in connective tissue. Collagen triggers fibroblast proliferation, support to form new granulation tissue and epithelium around wound. Wound treatment through collagen extract of Sangkuriang catfish skin can increase healing process. It stimulates humidity on level II burn injuries and encourage re-epithelization, proliferation and cell migration also increases growth. Methods: This is an experimental studies using 4 groups of Rattus Norvegicus (Wistar strain). All of the group get a burn injuries in their back skin. Control group 1 (K1) was treated by lidocaine for three days and control group 2 (K2) for ten days. Whereas treatment group 1 (P1) get lidocaine and collagen extract from Sangkuriang cat fish for 3 days and treatment group 2 (P2) for 10 days. Result: Number of fibroblast/field of view on treatment group showed significantly increased compared control groups on the 3rd days (P=0,046) and the 10th (P=0,004). Percentage of TNF-α on level II-burn injuries in wistar rats showed significant difference. Percentage of TNF-α lower than treatment group and showed significant lowering level compared control groups on the10th (P=0,022). Conclusion: Increasing of firoblast number and lowering level of TNF-α significantly showed collagen extract of Sangkuriang cat fish skin accelerate wound healing process.