Objective: The aim of this study was to determine the relative number of neutrophil (GR-1+) on mice infected Staphylococcus aureus after treatment noni (Morinda citrifolia L.) crude extract. Methods: Mice were devided into two group: non-infected and infected. Non infected group was without S.aureus infection whereas the infected group was infected with S.aureus. Group contain kontrol, dose 1 (25 mg/kg BW), dose 2 (100 mg/kg BW), and dose 3 (300 mg/kg BW). Oral treatment carried out for 20 days in every morning and each sample was injected with S.aureus at day 21 with 109 cell/mL. Relative number of neutrophil (GR-1+) was measured using the BD FACSCaliburTM Flowcytometer. Data were analyzed by using Analysis of Varians (p<0,05) and SPSS 16 for windows. Result: The result showed that administration of noni crude extract was significantly change the relative number of neutrophil (GR-1+). Conclusion: Treatment of noni (M. citrifolia) crude extract could increase relative amount of neutrophil (GR-1+) on infected S.aureus and without infected group. That might be caused by active compound on noni crude extract that can influence activity of increasing Granulocyte Colony Stimulating Factor (G-CSF) so can increase relative amount of neutrophil (GR-1+).