Abstract: Measurement LDL-Cholesterol (LDL-C) can be performed by estimating LDL-C (cLDL-C) in the blood. In addition to the first proposed Friedewald formula, several new formulas that promise better examination in determining LDL-C levels, proposed by Anandaraja, Puavilai, Chen, Vujovic, de Cordova and Dansethakul. The subjects of the study were patients who performed routine lipid profile examination in Institution of Clinical Pathology of Gedung Pusat Diagnostik Terpadu RSUD Dr. Soetomo Surabaya who performs 10 to 12 hours of fasting with TG less than 400 mg / dL. The sample consisted of 41 men and 48 women with an average age of 52 years. The statistical test on each cLDLC gave the Friedewald -1.32% bias value; Anandaraja -3,92%; Puavilai 4.26%, Chen -2.19%; Vujovic -23.65%; de Cordova -0.91% and Dansethakul 4.45%. The correlation coefficient on Friedewald 0.9509; Anandaraja 0.9013; Puavilai 0.9576, Chen 0.9585; Vujovic 0.8745; de Cordova 0.9300 and Dansethakul 0.9505. The proposed cLDL-C formula Chen et al promises in determining the LDL-C estimate and the Vujovic formula gives a poor result in this study. Validation methods should be applied to each of the cLDL formulas if they are to be applied to clinical laboratories.