Abstract: Type 2 diabetes is one of the most common chronic diseases in the world. It is estimated that more than 140 million people worldwide are currently suffering from diabetes, and by 2025, an estimated more than 300 million people will suffer from the disease. The purpose of this study was to evaluate the correlation of cholesterol with the incidence of Diabetes Mellitus disease. The type of this research is observational with cross-sectional analytic approach and done in PLN employees, total are 112 people of which are mens. All participants underwent biochemical analysis of fasting blood sugar (BSN) and 2 hours PP (post prandial), and total cholesterol level test. Determination of glucose by glucose oxidase method and cholesterol level by CHOD-PAP method. The mean results in normal cholesterol (< 200 mg/dl) obtained BSN = 73 mg / dl, 2 Hours PP = 92 mg / dl, and in abnormal cholesterol (> 200 mg/dl) mean BSN = 81 mg / dl, 2 hours PP = 109 mg / dl, in independent T test with p = 0001 in both groups of different cholesterol levels. Results of the Pearson's correlation test, there was a significant positive correlation between GDP and cholesterol levels (r = 0.262, p = 0.005), and 2 hours PP and cholesterol levels (r = 0.258, p = 0.006). Conclusions: There was a significant positive correlation between 2 hours PP and cholesterol levels. This study shows there is a correlation of cholesterol levels that develop diabetes mellitus type 2.