Contact dermatitis is an acute or chronic inflammatory reaction in the form of erythema, papular edema, vesicles, squares and itching caused by irritants or substances that attach on the skin. The purpose of this study is to determine the strong relationship between personal hygiene and the use of PPE in tobacco farmers with the incidence of contact dermatitis in tobacco farmers in the Ambulu village of Jember. This observational research had done by using a cross-sectional approach. This study utilizes a total population of 61 tobacco farmers.  The dependent variable in this study is the incidence of contact dermatitis which is experienced by tobacco farmers in the Ambulu village. While the independent variables are personal hygiene and the use of personal protective equipment (PPE). To discover the strong relationship between dependent and independent variables, there will be tested by statistical tests which applies the spss application and Chi-Square test. Resulting from the research, the relationship between personal hygiene and the incidence of contact dermatitis are categorized as a strong relationship with the point is 0.627. Whereas the use of PPE and the incidence of contact dermatitis obtains a result of 0.276 which is categorized as moderate relationships. As suggestions for tobacco farmers in Ambulu village is to pay attention to their personal hygiene and use Personal Protective Equipment while working.