Abstrak: Diet tinggi lemak dan fruktosa dapat menginisiasi terjadinya sindroma metabolik, diantaranya dislipidemia, hiperglikemia, dan resistensi insulin. Labu kuning dengan varietas Cucurbita Moschata diketahui memiliki kadar karotenoid yang tinggi, terutama α dan β-karoten, β-criptoxanthina, lutein, dan zeaxanthin. Penelitian ini menggunakan metode quasi eksperimental dengan rancangan post test only group design menggunakan tikus Sprague Dawley kedalam 5 kelompok (Kelompok kontrol normal : tikus sehat; Kelompok kontrol hiperglikemia: diet tinggi lemak & fruktosa (DTLF) ; Kelompok tepung labu kuning 0,16g/200g BB: DTLF + tepung labu kuning 0,16g/200g BB ; Kelompok tepung labu kuning 0,32g/200g BB: DTLF + tepung labu kuning 0,32g/200g BB; Kelompok tepung labu kuning 0,64g/200g BB: DTLF + tepung labu kuning 0,64g/200g BB). Pemberian diet tinggi lemak & fruktosa (DTLF) dilakukan selama 25 hari berturut-turut, selanjutnya dilakukan pemeriksaan kadar glukosa darah. Dilanjutkan pemberian tepung labu kuning dilakukan selama 4 minggu. Data dianalisis dengan uji parametrik yaitu one-way ANOVA dan uji t berpasangan dengan nilai probabilitas p<0,05. Hasil uji oneway ANOVA terhadap delta kadar GDP menunjukkan perbedaan yang bermakna antar kelompok (p<0,05), dan hasil uji post hoc didapatkan bahwa delta kadar GDP K3, K4, K5, dan K2 berbeda secara bermakna p<0,05. Hasil uji T berpasangan menunjukkan bahwa kadar GDP K2, K3, K4, dan K5 menunjukkan perbedaan yang bermakna setelah induksi DTLF dan setelah intervensi tepung labu kuning, sehingga dapat disimpulkan bahwa intervensi tepung labu kuning dapat menurunkan kadar GDP.Kata kunci : Sindroma metabolik, kadar glukosa darah, labu kuning, karotenoid.Abstract: High-fat and fructose diet which lasts longer be considered as a factor in the initiation of metabolic syndrome, likes dyslipidemia, hyperglycemia, and insulin resistance. Pumpkins with varieties Cucurbita Moschata is known have high levels of carotenoids, especially α & β-carotene, β-criptoxanthina, lutein, and zeaxanthin. This study used quasi experimental with post test only control group design. Twenty five male Sprague Dawley rats were induced by induced high fat and fructose diet. Rats were randomly divided into five groups: (1) normal control rats group, (2) hyperglicemia control rats groups, (3) HFFD rats receive powder pumpkin 0,16g/200g weight, (4) HFFD rats receive powder pumpkin 0,32g/200g weight, and (5) HFFD rats receive powder pumpkin 0,64g/200g weight. HFFD was given for 25 days and continued pumpkin powder was given for 28 days. Blood glucose levels were analyzed using glucose oxidase p-aminophenazone (GOD-PAP) method. Expression of IRS-1 genes were estimated using real time polymerase chain reaction (RT-PCR). Data analysis was performed with One way ANOVA/Post Hoc test and dependent t-test with significance level of 0.05. The oneway ANOVA test results on the delta of GDP levels showed significant differences between groups (p <0.05), and post hoc test results showed that the delta of K3, K4, K5 and K2 GDP differed significantly by p <0.05. Paired T-test results showed that the GDP of K2, K3, K4, and K5 showed significant differences after DTLF induction and after intervention of the pumpkin powder, so it can be concluded that the intervention of the pumpkin powder may decrease the GDP level.Keyword : Metabolic syndrome, blood glucose, cucurbita moschata, carotenoid.