Bali is a province in Indonesia that has a variety of uniqueness, one of which is the majority of people who follow the Hindu religion. Hinduism in Bali has Temple as a holy place to implement yadnya which aims to get closer to God Almighty of the many temples, there is a unique temple located in the village of Pakraman Payangandesa Gianyar. Taman Sari Temple is believed to be local people staying manifestation of God who as Dewi Manik Merta Sari. The worship of Dewi Manik Merta Sari Bead Goddess is a religious tradition that has a special uniqueness in Pakraman Payangandesa Village. Research on the existence, function and meaning of worship Dewi Manik Merta Sari in Taman Sari temple. The type of data used in this study is primary data sourced from the field and secondary data sourced from literature review, documents, articles, and lontar. Data were collected from the process of observation, interview, documentation study. To analyze the data in can use descriptive method and use several stages namely: data reduction, data display, and data verification. Then the data is presented with a qualitative discrete. Theories used include, religious theory, structural functional theory, symbol theory, and theory of existenceThe existence of Dewi Manik Merta Sari, which is located at Taman Sari temple is related to the history of Temple and the history of Pakraman Payangandesa Village. Dewi Manik Merta Sari is symbolized in the form of a statue or pralingga which is handled in palinggih Gedong Arca temple and ceremonial worship on buda wage klawu along with tradition in Taman Sari temple. Function of the Dewi Manik Merta Sari Goddess worship is the religious, fertility, and social. Worship of Dewi Manik Merta Sari Beads Goddess there are three meanings are explained namely: the meaning of theology, ethics, togetherness, and symbolic.