Wukirsari Village, Cangkringan District is belong to Merapi Mountain’s slopes which located between the Gendol River and Yellow River. Nowadays, we faced the problem of anorganic fertilizer overused such as Urea, ZA, TSP/SP-36 and KCl in agriculture land. The effort to return the soil organic compound can be done by added some organic compounds or microbial bio -organic fertilizer. Sludge is fermented biodigester yield and it has lost its gas. The aim of this research was to understand the physiological response and optimum dose of biogas as planting medium to ‘Segreng’ Rice planted in the rice field of Wukirsari Village, Cangkringan District, Sleman Regency. This research was done on greenhouse scale and rice field scale. The treatment given on 0; 1; 1,5; 2 and 2,5 liters per 100 m2 of rice field areas, and given on 0; 4; 8; 12; and 24 ml per 5 kg soil on polybags. Data were taken in three repetitions. The vegetative growth parameters included plant height, number of leaves, number of seedlings and chlorophyll content, while generative growth parameters measured included NRA levels, dried biomass including crown/stem, roots, filled grains, empty grains, and total weight and number of filled grains, empty rains, and the number of panicles. The result were tested with ONE WAY ANOVA (Analysis of Variance) with SPSS version 19 for Windows and followed by Duncan's Multiple Range Test with 95% significance level (α = 0.05). Generally, the result showed that biogas sludge can increase the vegetative and generative growth of rice plant ‘Segreng’ on polybag scale and rice field scale. The rice plant on polybag with 4 ml biogas sludge was significantly different on the vegetative growth and chlorophyll content, while the rice plant on polybag with 8 ml biogas sludge was significantly different on the generative growth and NRA levels.