Three experiments were conducted to evalu."'Ite the enzyme activity from rumen liquor in some feedstuffs and its effect on performance of broiler chickens fed cassava based-diet. Those were: enzyme activity test and degradation level; total solubility; and feeding trial. Ninety day old chicks Ross broiler were fed three experimental' diets i.e.: ration containing gaplek as control (R1); ration containing rumen liquor treated gaplek (R2) and R1 + 1 % Starbidf9 (R3) for 4 weeks on feeding trial.Results showed that enzyme activity of rumen liquor was 243 IU/g. Enzyme from rumen liquor was capable of degrading onggok (89.90%); diet containi..'''lg gaplek (87.05%); gaplek (85.89%); coconut meal (83.97%); rice straw (7L.79%J; and rice bran (33.75%). Total solubility of these feedstuffs also increased after the enzyme treatment. The total solubility of the feedstuffs were: rice bran (33.75%); onggok (29.24%); diet containing gaplek (23.80%); gaplek (14.02%); coconut oil meal (1289%); and rice straw (11.57%) respectively. It was concluded that the enzyme from rumen Ii~uor can improve feed quality of gaplek, but the improvement can not enhance feed conversion ratio and performance index oJ broiler chickens fed gaplek based-diet.