Diabetes mellitus is a disorder of carbohydrates, fats and proteins  metabolism which are shown by hyperglycemia due to a decrease of insulin secretion and sensitivity.  diabetes treatment can be done medically and traditionally. People often use the water of boiled plant  mangosteen rind, jengkol rind and black sticky rice traditionally. A research of anti diabetes mellitus water fraction activities test of black sticky rice was done to determine the proper dose of the water fraction of black sticky rice to decline blood glucose levels of diabetic mice. Blood glucose levels were determined by glucose tolerance method using an instrument called NESCO Multicheck Glukosa. Dose Variations used was 125 mg/kg, 250 mg/kg, 500 mg/kg of body weight and treatment times are 60, 90, 120, and 150 minutes. Research data were analyzed statisticaly by SPSS using analysis of variance (ANOVA) method and Duncan's multiple range test. The result showed that the water fraction of black sticky rice can decrease blood glucose levels of white mice. The value of  Fcalculated > Ftable which are 14.55 and 6.09 respectively. Water fraction dosage which cause a highest decrease of glucose levels was 500 mg/kg of body weight.