Plant roots host a variety of fungi. The association between fungi and plant roots categorized to mycorrhizal and non-mycorrhizal. One important group of non-mycorrhizal symbioses is the dark septate endophytes (DSE) that colonize plant roots and form characteristic intracellular structuresmelanized hyphae and microsclerotia. Compared to better known mycorrhizal symbioses or the vertically transmitted systemic foliar endophytes, the DSE have received very little attention. So, the research was conduct to analize the characteristics of DSE in roots system of maize and rice plant. The roots sample was taken from maize and rice fields around Kuranji District, Padang. Occurance of DSE was observe by tryphan blue staining (0.05%) using digital microscope. DSE was found in 65% of the total of maize root segments observed and 30% for roots of rice plant.  DSE occurs in corn roots system as brown hyphae inter-and intracellularly and dark-brown microsclerotia intracellularly. The smaller microsclerotia found in rice roots system and absorbed tryphan blue, then appeared as blue color. The melanized hyphae found in rice roots system was different with those found in maize root.