This study aims to determine (1) The influence of interest on student learning outcomes in Biology science lessons in SMP Negeri 2 Jayapura, (2) The influence of the use of methods and interests on student learning outcomes in the science of Biology in SMP Negeri 2 Jayapura, (3) interaction between method and interest toward student learning outcomes in science biology lesson in SMP Negeri 2 Jayapura. This research type is experiment. This research took place from February to May 2017. The population of this study is all students of class VII in SMP Negeri 2 Jayapura. The sample of this research is all students of class VIIJ, VIIK, and VIIL which is divided into 4 classes. Data analysis uses prerequisite test with parametric analysis path with normality test, homogeneity test, and interest question variable data. While hypothesis testing using ANAVA two way. The results showed (1) There is an influence of interest that arise from within the students to the learning outcomes achieved in the learning process with the results of the calculation of high interest variable average 74.87 and the low interest variable average 65.04. (2) There is an average influence of Biology Science learning outcomes in students who are taught using Student Worksheet (LKS) Word Square Model from the students who taught using lecture method on students who have high interest result Fcount = 2.967 more than Ftable = 2.46. (3) There is an average influence of Biology Science learning outcomes in students who are taught using Student Worksheet (LKS) Word Square Model from the students taught using lecture method on students who have low interest result Fcount = 2.952 more than Ftable = 2.46. (4) There is a very significant influence between the learning method and the interest or the influence of the method on the learning outcomes of Biology IPA depending on the student's interest result Fcount = 3.776 more than Ftable = 2.46. Keywords: word square, interests, learning outcomes