Objective of this study was to determine the effect of prayer on pre-hemodialysis blood pressure of CRF patients in the hemodialysis room of the Jakarta Islamic Hospital. Data collection was done through purposive sampling. This study examined 32 respondents. The results of the study indicated there was a decrease in blood pressure in the intervention and control groups before and after giving prayers on systolic and diastolic blood pressure. The results of the paired t-test before and after being given prayers in the intervention and control groups produced a sig value of <0.05 in systolic and diastolic blood pressure, which means that there were differences before and after giving prayers. The unpaired t-test results in the intervention and control groups of systolic blood pressure produced a sig value> 0.05, which means that there was no difference between the intervention and control group. On the contrary, diastolic blood pressure produced a sig value <0.05, which means that there were differences between the intervention and control group. Finally, it can be concluded that the provision of a prayer intervention in pre-hemodialysis was effectively proven to reduce blood pressure. Keywords : chronic kidney disease, blood pressure, prayer.