Aedes aegypti mosquito, which acts as a vector in the spread of dengue disease. One of the effort to reduce the spread of dengue disease is using larvicides. Brotowali thought to have an effect as larvicides against Aedes aegypti larvae because they have the alkaloid compounds and tinokrisposid that can cause death in larvae. This study aims to determine the effect extract brotowali (Tinospora crispa) against Aedes aegypti larvae of death so as to provide information to the public that can be used as larvicidal brotowali. This research is an experiment with a completely randomized design (CRD) with 4 treatments and 6 replicates. The samples used were third instar larvae of Aedes aegypti with the treatment A0 (abate 500 ppm), A1 (500 ppm), A2 (750 ppm), and A3 (1000 ppm). Observations dead larvae were carried out for 24 hours and 48 hours. The results showed that within 24 hours at a concentration of 500 ppm on average larvae death reached 32.5%, the concentration of 750 ppm 55.8%, a concentration of 1000 ppm to 70%, whereas within 48 hours at a concentration of 500 ppm on average larvae death reached 75%, 90.8% concentration of 750 ppm, a concentration of 1000 ppm to 100%. Based on analysis of variance (Ansira) showed that the extract brotowali (Tinospora crispa) gives a significant influence (p > 0.01) in the death of larvae of Aedes aegyti. Effective concentration to kill larvae of Aedes aegyti namely the concentration of 750 ppm.