Extract of Dragon Fruit Pulp (Hylocereus polyrhizus) Potentially Stain Chromosomes of Red Onion (Allium ascalonicum) (In press)

Abstract

Side effect of aceto-orcein as a dye of chromosome structures remains some risks. This dye may remain tissue damage on eyes mucous membrane, burnt by skin contact, severe irritation of respiratory by spray mist. Exploration of natural dye is an alternative solution. Betacyanin of red dragon fruits Hylocereus polyrhizus pulp might have a potential role to stain chromosome. Aim of this study is to extraction of dragon fruit pulp (Hylocereus polyrhizus) to stain chromosomes of mitotic cells of red onion Allium ascalonicum. The method used in this research is qualitative description by using squash method with aceto-orcein as synthetic dye and two treatments of extract ratio of red dragon fruits pulps as natural dye. The result revealed that 2:1 ratio of red dragon fruits and distilled water could stain chromosomes of red onion somatic cells than 1: 1 ratio of red dragon fruits and distilled water. Further specific solvent is required to further investigation to get best betacyanin extract of red dragon fruits pulps.