The objectives of this study are: (1) to know the need of teaching materials for the senior high school teachers and the students, (2) to know the procedures of Biology teaching materials development of contextual teaching learning-based teaching, (3) to develop Biology teaching materials of contextual teaching learning-based teaching. The research method of this study is research and development. This study found: <em>firstly</em>, the need of teaching materials for the senior high school teachers and the students considers some elements, they are a) situational context, b) fits to curriculum, c) exercises/tasks enable to activate the students’ achievement, d) practical based implementation, and e) interesting, clear, and easy to understand. <em>Secondly</em>, the model of development is adapted from Borg and Gall, they are: data collection, planning, drafting a model, experimenting the model, revision the draft, field experiment, revising the final draft, and implementation. <em>Thirdly</em>, the score of post test shows 66.9 (control class) and 79.6 (experimental class), t-value of f value is 2.67 &gt; 1.67 of f table. It can be concluded that the use of Biology CTL based-teaching materials enables to improve the students’ achievement in learning Biology.