A human movement as a response of something is always different for each  individual. However, the response is often difficult to measure because it is very flexible and sometimes are very subjective. Research was conducted with the goal to design and create tools measure the response speed of human-based mikrokontroller AT89S8252 and determine the level of accuracy tool. Measu rement tool consists of 4 switch, ie, 2, and for tester 2 for participants who tested, 2 indicator LED, buzzer, 4 buttons (menu, enter, select and cancel), speaker, and MK AT89S8252. LCD as a display. For the data on the motor response and eye response, motor ears done 9 times as much for each individual. Before the test on the motor response, eye and ear motor is done, first time on the tool dikalibrasikan using the time on the stopwatch 1 / 1000. After that the process of  testing carried out by determining the response to be conducted, the examiner pressing switch S1 or S2 switch, then LED1 or LED2 akan buzzer or light simultaneously. Participants must be tested first with a quick switch press switch S3 or S4 to stop the buzzer and the process of calculation. Step response of the data on the eyes and ears are the same. In the process of calibration equipment with time using the stopwatch 1 / 1000 data obtained 9. The relative error deviation of 2.5% was obtained, the accuracy produced by means of measuring the speed of human response, 97.5%. Boundary condition is the human response that is the limit of 3 is very good (one limit), normal / good (limit two), poor (limit three). In the testing of motor response in humans that use LED indicator, the data better than a motor ear response data, is due to the rapid Rambat different. LED light will reach the human eye without the lapse of time, but the sound or the sound will reach the human ear after the time interval. Kata Kunci:Kecepatan, Gerak, Respon, Mikrokontroller