The purpose of this study is to find out how the process of developing E- Modules is assisted by Flip PDF Professional in Biochemistry courses with the subject matter of Carbohydrate in order to help students understand the material Carbohydrates, overcome the limitations of time and lack of facilities and infrastructure and help students in independent study. The development model chosen is ADDIE. The test subjects in this study were is 2 experts to determine the level of validity of the developed product and 18 students to determine the level of practicality of the product being developed. This study uses a collection data consisting of expert validation sheets and student response questionnaires. The results show that the developed E-module is valid and practical, with an average level of validity from media and material experts is 82.5% in the "very valid" category. The results of the practicality test based on the assessment of the student response questionnaire obtained an average percentage of 90.1% with the practicality criterion of "very practical". Thus, it can be concluded that the Biochemistry courses with the subject matter of Carbohydrate has been tested for validity and practicality.